Concentration Assay:Article Title: Lipase enzyme
Article Snippet: .. Media: LB (Lysogeny Broth) medium 10 g/L casein peptone 5 g/L yeast extract 5 g/L g/L NaCl In distilled water, pH 7.5 LB agar plates 15 g/L Agar-agar In LB medium optional 25 μg/mL kanamycin or 100 μg/mL ampicillin Cre-Lox - LB agar plates 7% (w/v) Sucrose 30 μg/mL Chloramphenicol 100 μg/mL Ampicillin 86% (v/v) LB with 15 g/L agar Super Optimal Broth (SOC) medium, Invitrogen GmbH, Germany, Karlsruhe Dryls (1x) 1.5 mM Trinatriumcitrat-Dihydrat 1 mM NaH2PO4-Monohydrat 1 mM Na2HPO4 1.5 mM CaCl2 MW 1010 10 g/L malt extract 10 g/L Wheat peptone E1 5 g/L yeast extract 2 g/L glucose monohydrate 1 mL/L ferrous-sulphate/chelate solution MW 1515 15 g/L malt extract 15 g/L Wheat peptone E1 5 g/L yeast extract 2 g/L glucose monohydrate 1 mL/L ferrous-sulphate/chelate solution Paromomycin concentration for Fermentation: 224 μg/ml Paromomycin Paromomycin concentration for adaption of mutants: 196 μg/ml Paromomycin Buffers As buffers the typical buffers used in hydrophobic interaction chromatography are being applied, as e.g. disclosed in Djogo et al 1999, the content of which is incorporated by reference herein. .. Machines and Columns Equipment Designation Supplier Äkta Pure and Chromatography GE Healthcare D-A-CH, Fraction collector system Munich, Germany F9-C Biolistic gun PDS-1000/He BioRad Laboratories GMBH Biolistic ® Particle Hercules-California, USA Delivery System BioSpectrometer Absorption Eppendorf AG, Hamburg, kinetic measurement Germany Capto Octyl HIC Column GE Healthcare D-A-CH, Munich, Germany Fusion Solo Fluorescent Vilber Lourmat Deutschland measurement GmbH, Eberhardzell, Germany Mini Protean Electrophoresis BioRad Laboratories GMBH Tetracell Chamber Hercules-California, USA Multiforce HAT 1 l Bioreactor Infors HT, Bottmingen-Basel Switzerland Superdex Increase SEC Column GE Healthcare D-A-CH, 10/300 GL Munich, Germany ThermoMixer Tempering and Eppendorf AG, Hamburg, mixing samples Germany 1.
Hydrophobic Interaction Chromatography:Article Title: Lipase enzyme
Article Snippet: .. Media: LB (Lysogeny Broth) medium 10 g/L casein peptone 5 g/L yeast extract 5 g/L g/L NaCl In distilled water, pH 7.5 LB agar plates 15 g/L Agar-agar In LB medium optional 25 μg/mL kanamycin or 100 μg/mL ampicillin Cre-Lox - LB agar plates 7% (w/v) Sucrose 30 μg/mL Chloramphenicol 100 μg/mL Ampicillin 86% (v/v) LB with 15 g/L agar Super Optimal Broth (SOC) medium, Invitrogen GmbH, Germany, Karlsruhe Dryls (1x) 1.5 mM Trinatriumcitrat-Dihydrat 1 mM NaH2PO4-Monohydrat 1 mM Na2HPO4 1.5 mM CaCl2 MW 1010 10 g/L malt extract 10 g/L Wheat peptone E1 5 g/L yeast extract 2 g/L glucose monohydrate 1 mL/L ferrous-sulphate/chelate solution MW 1515 15 g/L malt extract 15 g/L Wheat peptone E1 5 g/L yeast extract 2 g/L glucose monohydrate 1 mL/L ferrous-sulphate/chelate solution Paromomycin concentration for Fermentation: 224 μg/ml Paromomycin Paromomycin concentration for adaption of mutants: 196 μg/ml Paromomycin Buffers As buffers the typical buffers used in hydrophobic interaction chromatography are being applied, as e.g. disclosed in Djogo et al 1999, the content of which is incorporated by reference herein. .. Machines and Columns Equipment Designation Supplier Äkta Pure and Chromatography GE Healthcare D-A-CH, Fraction collector system Munich, Germany F9-C Biolistic gun PDS-1000/He BioRad Laboratories GMBH Biolistic ® Particle Hercules-California, USA Delivery System BioSpectrometer Absorption Eppendorf AG, Hamburg, kinetic measurement Germany Capto Octyl HIC Column GE Healthcare D-A-CH, Munich, Germany Fusion Solo Fluorescent Vilber Lourmat Deutschland measurement GmbH, Eberhardzell, Germany Mini Protean Electrophoresis BioRad Laboratories GMBH Tetracell Chamber Hercules-California, USA Multiforce HAT 1 l Bioreactor Infors HT, Bottmingen-Basel Switzerland Superdex Increase SEC Column GE Healthcare D-A-CH, 10/300 GL Munich, Germany ThermoMixer Tempering and Eppendorf AG, Hamburg, mixing samples Germany 1.
Saline:Article Title: Rapid identification of new isolates of Acidipropionibacterium acidipropionici by fluorescence in situ hybridization (FISH).
Article Snippet: .. The milk and manure amples were serially diluted in phosphate-buffered saline 130 mmol/l NaCl, 10 mmol/l sodium phosphate buffer, pH .4) (PBS) and plated onto modified Lactate agar [24 ml/l odium lactate (60% v/v), 30 g/l casein peptone, 30 g/l yeast xtract, 125 mM lithium chloride and 15 g/l agar, pH 6.8]. s D d he plates were incubated for ten days at 37 ◦C in an naerobic atmosphere provided by Anaerocult A (Merk, Gerany) in an anaerobic jar (AnaeroGen system, Oxoid, UK). onvex and punctual colonies with creamy texture were ransferred to LAPTg agar and incubated at 37 ◦C for ten ays in anaerobic conditions. ..
Modification:Article Title: Rapid identification of new isolates of Acidipropionibacterium acidipropionici by fluorescence in situ hybridization (FISH).
Article Snippet: .. The milk and manure amples were serially diluted in phosphate-buffered saline 130 mmol/l NaCl, 10 mmol/l sodium phosphate buffer, pH .4) (PBS) and plated onto modified Lactate agar [24 ml/l odium lactate (60% v/v), 30 g/l casein peptone, 30 g/l yeast xtract, 125 mM lithium chloride and 15 g/l agar, pH 6.8]. s D d he plates were incubated for ten days at 37 ◦C in an naerobic atmosphere provided by Anaerocult A (Merk, Gerany) in an anaerobic jar (AnaeroGen system, Oxoid, UK). onvex and punctual colonies with creamy texture were ransferred to LAPTg agar and incubated at 37 ◦C for ten ays in anaerobic conditions. ..
Incubation:Article Title: Rapid identification of new isolates of Acidipropionibacterium acidipropionici by fluorescence in situ hybridization (FISH).
Article Snippet: .. The milk and manure amples were serially diluted in phosphate-buffered saline 130 mmol/l NaCl, 10 mmol/l sodium phosphate buffer, pH .4) (PBS) and plated onto modified Lactate agar [24 ml/l odium lactate (60% v/v), 30 g/l casein peptone, 30 g/l yeast xtract, 125 mM lithium chloride and 15 g/l agar, pH 6.8]. s D d he plates were incubated for ten days at 37 ◦C in an naerobic atmosphere provided by Anaerocult A (Merk, Gerany) in an anaerobic jar (AnaeroGen system, Oxoid, UK). onvex and punctual colonies with creamy texture were ransferred to LAPTg agar and incubated at 37 ◦C for ten ays in anaerobic conditions. ..
|
Thermo Fisher is a verified supplier 
Thermo Fisher manufactures this product 